STUDY OF INTERACTIONS IN MONO- AND POLYANTIGEN VACCINES IN ANIMALS (IN VIVO)

Abstract

The article presents the results of an experimental study on antigen interactions during the administration of mono- and polyantigenic vaccine preparations under in vivo conditions. It was shown that combined antigenic preparations exhibit higher immunogenicity. The most pronounced immune response was observed when modern adjuvants POLYGEN and Montanide ISA 71 were used. The obtained results confirm the prospects for the development of associated vaccines for the prevention of infectious diseases in animals.

The aim of the present study was to investigate antigen interactions under in vivo conditions in order to assess manifestations of synergism and antagonism when mono- and polyantigenic vaccine preparations are used in veterinary medicine. Experimental studies were carried out on laboratory animals using different variants of associated inactivated vaccines containing various adjuvants. Sterility, safety, and antigenic activity of the preparations were evaluated, as well as the dynamics of antibody production.

The results showed that polyantigenic vaccines are capable of inducing a more pronounced immune response compared to monoantigenic preparations. The highest antibody levels were observed when vaccines containing the polymer adjuvant POLYGEN and the oil adjuvant Montanide ISA 71 R VG were used. At the same time, certain antigen combinations led to a decrease in the immune response, indicating the presence of antagonistic interactions. It was established that the proper selection of antigens and adjuvants significantly increases vaccine immunogenicity and contributes to the formation of a stable humoral immune response. The obtained results confirm the prospects of using polyantigenic vaccines and hyperimmune sera in veterinary practice for the prevention of infectious diseases in animals.