Biocompatibility Assessment of Herbal Components of The Novel Solid Ointment

Abstract

The novel cosmetic solid ointment was created based on the composition of the plant substance Melilot (hereinafter referred to as Melilot) and biocompatible, biodegradable amino acid polymer-film-forming pseudoproteins [1,2,3], which is effective for the prevention of any kind of scars or spots after plastic surgery and enhancing the effect of cosmetic procedures.

The ointment will contribute to the formation of an invisible "cosmetic" scar after plastic surgery and protect the skin from the formation of normotrophic, atrophic, hypertrophic, keloid scars, brown and red spots, and itching during the wound healing process. It can also be used after any cosmetic procedures, such as mechanical, chemical, enzymatic and physical peeling etc, to prevent unwanted results and enhance the effect of these procedures.

Melilot, a component of solid ointment, has been studied for its acute toxicity and local skin irritation. Melilot is a 2.84% composite and consists of the following plant substances: Maclura pomifera (Raf.) G.K. Schneid, Ghelidonium majus L., Trifolium pretense L., Mellilotus officinalis L., Juglans regia L.

The study was conducted at the TSU Alexander Natishvili Institute of Morphology, on 12 adult male Wister albino rats with a body weight of 200–250 grams.

 The animal care protocol followed the updated recommendations of the National Research Council (USA) Committee - "Guide for the Care and Use of Laboratory Animals", to minimize animal pain and/or discomfort, both during manipulation and animal sacrifice after the experiment [4];

Animals were kept under comfortable laboratory conditions (22 °C, 12 h light/dark cycle, 60% humidity, free access to food and water) before and throughout the experiment.